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t-Virus (short for "Tyrant Virus") was the collective name for a series of artificial mutagen RNA viruses designed as weaponized variants of the Progenitor Virus for military application. They inherited the DNA mutation property of the Progenitor Virus and drastically improved on it. The t-Virus is capable of inducing sudden mutations on a host and is the raw material most suitable for biological weapons creation. Upon infection, it rewrites DNA and mutates the host into a creature with different properties and abilities which cannot be found in the natural world.

Albert Wesker was one of the most promising subjects of the Wesker Project. Following his education, he became a Chief Researcher and prospective executive of Umbrella Corporation. He was an expert in bioengineering and became directly involved with research on the t-virus. He later became the leader of S.T.A.R.S. as Alpha Team Captain in Raccoon City.

Raccoon City was an urban community located in the American mid-west. At its peak, it had a population of over one hundred thousand. While existing as a town initially, it was thanks to the many donations by Umbrella that Raccoon prospered into a full fledged city in the late 20th century. Following the T-virus outbreak that afflicted the city in September 1998, the entire community was destroyed by an experimental nuclear weapon by the United States government, as part of Mission Code: XX (Bacillus Terminate).

Jill Valentine was a former member of the Special Forces S.T.A.R.S. (Special Tactics and Rescue Service) of the Raccoon City police. She had years of tactical experience and certification and had received training in a training program of the U.S. Army Delta Force where she earned high grades in bomb disposal, making her an extremely competent and experienced member of any unit.