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The t-virus (short for "Tyrant Virus") was a highly adaptable bioweapons-grade pathogen and the main component for the Umbrella Corporation in their biological weapons development program, created from an attempt to weaponize the Progenitor virus for military use and application. The t-virus became the basis for the majority of Umbrella's research. It inherits the genetic modifying properties of the Progenitor virus and drastically improves on them. The very first strain of the t-virus was created by Dr. James Marcus.

Alexia Ashford was a brilliant scientist and the younger twin sister of Alfred Ashford and the successful result of the "CODE:Veronica Project" initiated by Alexander Ashford. She was a clone of Veronica Ashford and considered to be her reincarnation, inheriting her beauty and even surpassing her in intelligence, factors which reminded others of Veronica. To others, she is known as the creator of the t-Veronica virus.

The Spencer Rain was a luxury passenger cruise liner owned and operated by the Umbrella Corporation. It was christened after the founder, CEO and President of Umbrella; Ozwell E. Spencer.

"Discipline, Obedience, Loyalty" ~ Candidate Motto

The Umbrella Research Center served as one of the first facilities constructed by the Umbrella Corporation. Its purpose was to educate prospective officers and executives of Umbrella, but it also served as the private laboratory of James Marcus. It was also the birthplace of the t-virus. It is also commonly known as the "Management Training Facility".

The Arklay Laboratory was the first of many clandestine biological weapon research laboratories to be established by the Umbrella Corporation which operated as an annexed laboratory of Umbrella U.S.A. The actual facility was hidden beneath a large European-style mansion estate built in the backwoods of Raccoon Forest in the Arklay Mountains on the outskirts of Raccoon City. Its design was based partially on one of the many residences of Oswell E. Spencer; the Spencer Estate.