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The t-virus (short for "Tyrant Virus") was a highly adaptable bioweapons-grade pathogen and the main component for the Umbrella Corporation in their biological weapons development program, created from an attempt to weaponize the Progenitor virus for military use and application. The t-virus became the basis for the majority of Umbrella's research. It inherits the genetic modifying properties of the Progenitor virus and drastically improves on them. The very first strain of the t-virus was created by Dr. James Marcus.

Jill Valentine was a former member of the Special Forces S.T.A.R.S. (Special Tactics and Rescue Service) of the Raccoon City police. She had years of tactical experience and certification and had received training in a training program of the U.S. Army Delta Force where she earned high grades in bomb disposal, making her an extremely competent and experienced member of any unit.

The Arklay Laboratory was the first of many clandestine biological weapon research laboratories to be established by the Umbrella Corporation which operated as an annexed laboratory of Umbrella U.S.A. The actual facility was hidden beneath a large European-style mansion estate built in the backwoods of Raccoon Forest in the Arklay Mountains on the outskirts of Raccoon City. Its design was based partially on one of the many residences of Oswell E. Spencer; the Spencer Estate.

Trevor's Notes are scrapped in-game files from BIO HAZARD 1, which were written by the architect of the mansion, George Trevor.

The Crow as an irregular mutant first surfaced in the Arklay Mountains, having been exposed to the t-virus. Since then, it is a common sight in many areas where a biohazard caused by the virus is prevalent.